Glycolate oxidase content of microbodies as affected by nitrate.
نویسندگان
چکیده
Glycolate oxidase is loosely held by microbodies obtained from etiolated barley (Hordeum vulgare L.) leaves depleted of nitrate. Defined centrifugation conditions cause the complete detachment of the enzyme from the microbodies. Addition of nitrate to these plants brings about a greater retention of glycolate oxidase by the microbodies. Synthesis of a nitrate-induced protein seems to be responsible for the enhanced retention of glycolate oxidase. Catalase, on the contrary, is strongly attached to the microbodies under all nutritional and experimental conditions considered.
منابع مشابه
Localization of Enzymes within Microbodies
Microbodies from rat liver and a variety of plant tissues were osmotically shocked and subsequently centrifuged at 40,000 g for 30 min to yield supernatant and pellet fractions. From rat liver microbodies, all of the uricase activity but little glycolate oxidase or catalase activity were recovered in the pellet, which probably contained the crystalline cores as many other reports had shown. All...
متن کاملPeroxisomes from spinach leaves containing enzymes related to glycolate metabolism.
Microbodies, designated as peroxisomes because of their enzyme complement, have been isolated from spinach leaves. After grinding leaves in 0.5 M sucrose, the peroxisomes were removed with the broken chloroplast fraction by differential centrifugation. During sucrose density gradient centrifugation, the peroxisomes banded in about 1.9 M sucrose and were separated from mitochondria and chloropla...
متن کاملInvestigation of the glyoxysome-peroxisome transition in germinating cucumber cotyledons using double-label immunoelectron microscopy
Microbodies in the cotyledons of cucumber seedlings perform two successive metabolic functions during early postgerminative development. During the first 4 or 5 d, glyoxylate cycle enzymes accumulate in microbodies called glyoxysomes. Beginning at about day 3, light-induced activities of enzymes involved in photorespiratory glycolate metabolism accumulate rapidly in microbodies. As the cotyledo...
متن کاملMicrobodies (Glyoxysomes and Peroxisomes) in Cucumber Cotyledons: Correlative Biochemical and Ultrastructural Study in Light- and Dark-grown Seedlings.
The changes in activities of glyoxysomal and peroxisomal enzymes have been correlated with the fine structure of microbodies in cotyledons of the cucumber (Cucumis sativus L.) during the transition from fat degradation to photosynthesis in light-grown plants, and in plants grown in the dark and then exposed to light. During early periods of development in the light (days 2 through 4), the micro...
متن کاملاثر منبع نیتروژن بر برخی صفات فیزیولوژیکی و بیوشیمیایی لوبیا در شرایط تنش کم‌آبی
Nitrogen has an important role for supplying carbon skeletons which needs for producing compatible solutes and stress tolerance enzymes. Water deficit affects nitrogen fixation in legums. So we studied the effect of various nitrogen sources on physiological, and biochemical features of bean under water stress. This experiment was conducted as factorial based on completely randomized design with...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 55 2 شماره
صفحات -
تاریخ انتشار 1975